#CLC GENOMICS WORKBENCH 9 MAC OS#

The red dots indicate the number of significantly up- and down-regulated genes using p < 0.01 and ☒-fold change as the cutoff threshold.

In the plot, log 2 of fold change between the malE and CBP dsRNA-treated insects are plotted against −log10 (p), where p is a probability value (for a given gene) that is associated with the EDGE comparison of the two groups of samples. Single nucleotide polymorphism (SNP), multiple nucleotide variation (MNV), and small insertions or deletions (InDel) were detected using CLC Genomics Workbench.

c Volcano plot of expression data after EDGE analysis. The CLC system requirements are provided online. b Hierarchical clustering based on the overall gene expression pattern showing the relative differences within and among the biological replicates from malE or CBP dsRNA injected larvae. CLC Genomics Workbench 21.0.4 and CLC Microbial Genomics Module (CLC MGM) 21.0 (Redwood City, CA, USA) were installed on an HP notebook computer (specifications: Windows 10 operating system, Intel i7-7500U dual-core processor 2.70 GHz and 8 GB RAM) for all analyses. The color spectrum, stretching from blue to red, represents TMM normalized expression values obtained after EDGE analysis. a Heatmap showing the overall gene expression pattern of all four biological replicates from malE or CBP dsRNA injected larvae. Cutting-edge technology The workbench incorporates cutting-edge technology and the newest state-of-the-art algorithms, while also supporting and integrating into the rest of a typical NGS workflow.

#CLC GENOMICS WORKBENCH 9 MAC OS#

Gene expression analysis of RNA isolated from dsmalE (control, M1-M4) and dsCBP injected (C1-C4) last instar Tribolium larvae using the empirical analysis of DGE (EDGE) algorithm in CLC Genomics Workbench (Version: 9.5.9) and WEGO. CLC Genomics Workbench is a cross-platform desktop application and is compatible with Windows, Mac OS X, and Linux platforms.

Clc genomics workbench 9